{"id":1950,"date":"2025-09-28T19:01:55","date_gmt":"2025-09-28T17:01:55","guid":{"rendered":"https:\/\/biomed-center.com\/?page_id=1950"},"modified":"2025-10-08T18:54:54","modified_gmt":"2025-10-08T16:54:54","slug":"go3d","status":"publish","type":"page","link":"https:\/\/biomed-center.com\/en\/go3d\/","title":{"rendered":"GO>3D Bioreactor System"},"content":{"rendered":"<div class=\"kb-row-layout-wrap kb-row-layout-id1950_6b63c8-20 alignfull has-theme-palette8-background-color kt-row-has-bg wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-2-columns kt-row-layout-equal kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-middle\">\n\n<div class=\"wp-block-kadence-column kadence-column1950_1e279e-10 kb-section-dir-vertical inner-column-1\"><div class=\"kt-inside-inner-col\">\n<h2 class=\"kt-adv-heading1950_ea688a-7f wp-block-kadence-advancedheading\" data-kb-block=\"kb-adv-heading1950_ea688a-7f\">The Go&gt;3D System: a versatile tool for tissue culture<\/h2>\n\n\n\n<p class=\"kt-adv-heading1950_5efc4e-6f wp-block-kadence-advancedheading has-theme-palette-4-color has-text-color\" data-kb-block=\"kb-adv-heading1950_5efc4e-6f\">Classical 2D cell culture is important for many standard procedures, but real cellular behavior takes place in a 3D-environment. Step up to more realistic in vitro tissue models by using perfusion and mechanical stimulation in your routine culture. GO&gt;3D provides you with easy to use, versatile and robust systems that easily integrate into lab life to enhance your cell culture workflow. Made from highest quality PEEK implant material, galvanized aluminum and medical standard connectors and tubing, GO&gt;3D is built to last and survive many cycles of cleaning and autoclaving. Since we work in research ourselves, we have realized this at a price that fits into funding budgets, by serializing production of a design that is open for many different settings and modifications. With statistical analysis in mind, every unit features four wells for simultaneous sample incubation, transferring directly from a column of standard tissue culture plates. Enter the fascinating world of three dimensions in tissue culture, discover how easy it is to GO&gt;3D!<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_8f2b14-26 inner-column-2\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-kadence-image kb-image1950_dd3332-05 kb-image-is-ratio-size\"><div class=\"kb-is-ratio-image kb-image-ratio-square\"><img loading=\"lazy\" decoding=\"async\" width=\"1296\" height=\"864\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial.jpg\" alt=\"GO&gt;3D bone scaffolds perfusion\" class=\"kb-img wp-image-1906\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial.jpg 1296w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-300x200.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-1024x683.jpg 1024w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-768x512.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-500x333.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-800x533.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-1280x853.jpg 1280w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Standardmaterial-850x567.jpg 850w\" sizes=\"auto, (max-width: 1296px) 100vw, 1296px\" \/><\/div><\/figure>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n\n\n<figure class=\"wp-block-video\"><video controls src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/Go3D-Presentation-2025-BioMed-Center-Innovation.mp4\"><\/video><\/figure>\n\n\n\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-1950_d97128-76\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 style=\"text-align: center;\" class=\"advgb-dyn-5296689f\">Physiology in 3D Cell Culture<\/h2>\n\n\n<div class=\"kb-row-layout-wrap kb-row-layout-id1950_10f574-69 alignnone wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-2-columns kt-row-layout-equal kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-top\">\n\n<div class=\"wp-block-kadence-column kadence-column1950_fadc6b-07\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"378\" height=\"152\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_1.png\" alt=\"physiological processes in 3D\" class=\"wp-image-1911\" style=\"width:420px;height:auto\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_1.png 378w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_1-300x121.png 300w\" sizes=\"auto, (max-width: 378px) 100vw, 378px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_c5e3bd-b5\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"378\" height=\"206\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_2.png\" alt=\"physiological gradients in 3D\" class=\"wp-image-1910\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_2.png 378w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/physiological_gradients_2-300x163.png 300w\" sizes=\"auto, (max-width: 378px) 100vw, 378px\" \/><\/figure>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<p>The metabolism of the cells produces a gradient of oxygen, nutrients and pH in the scaffold. This leads to a marked gradient of cellular vitality and activity from the edges towards the center and ultimately to the death of cells towards the center. Thus, when measuring activity of ATDC5 cells in 3D scaffolds after 1 week of culture, the total activity decreases when edge\/middle ratio becomes lower.<\/p>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"722\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-scaled.jpg\" alt=\"Grafik showing vitality of cell dependent on fraction of edge\" class=\"wp-image-1915\" style=\"object-fit:cover\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-scaled.jpg 2560w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-300x85.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-1024x289.jpg 1024w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-768x217.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-1536x433.jpg 1536w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-2048x577.jpg 2048w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-500x141.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-800x226.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-1280x361.jpg 1280w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-1920x541.jpg 1920w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/vitality-fractions-850x240.jpg 850w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><\/figure>\n\n\n\n<h4 style=\"text-align: center;\">The \u201e500\u00b5m-Boundary\u201c: diffusion in 3D-scaffolds is very limited<\/h4>\n\n\n<div class=\"kb-row-layout-wrap kb-row-layout-id1950_4aac2a-ce alignnone wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-row-layout-equal kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-top\">\n\n<div class=\"wp-block-kadence-column kadence-column1950_a9cb35-4f\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"984\" height=\"779\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD.jpg\" alt=\"500mu boundary in alginate scaffold\" class=\"wp-image-1917\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD.jpg 984w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD-300x238.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD-768x608.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD-500x396.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD-800x633.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Alginate_LD-850x673.jpg 850w\" sizes=\"auto, (max-width: 984px) 100vw, 984px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_1b0a66-63\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"984\" height=\"779\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD.jpg\" alt=\"500mu boundary in chitosan scaffold\" class=\"wp-image-1916\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD.jpg 984w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD-300x238.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD-768x608.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD-500x396.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD-800x633.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_LD-850x673.jpg 850w\" sizes=\"auto, (max-width: 984px) 100vw, 984px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_3b2bb1-31\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"448\" height=\"355\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_HE.jpg\" alt=\"500mu boundary in chitosan scaffold HE\" class=\"wp-image-1918\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_HE.jpg 448w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/Chitosan_HE-300x238.jpg 300w\" sizes=\"auto, (max-width: 448px) 100vw, 448px\" \/><\/figure>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n<p class=\"has-small-font-size\">ATDC5 after 14d culture in 2% alginate (left) and chitosan-scaffolds (middle); calcein AM stain, right: H.E. in paraffine thin section<\/p>\n\n\n\n<p>The same observation in different static cell 3D cell cultures: in the first 500\u00b5m, cells thrive, then further inward they do not develop. Diffusion measurement experiments confirm the extremely low rate of static diffusion. For vital 3D cell cultures, perfusion or even compression is needed.<\/p>\n\n\n<div class=\"kb-row-layout-wrap kb-row-layout-id1950_0ac654-57 alignnone wp-block-kadence-rowlayout\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-row-layout-equal kt-tab-layout-inherit kt-mobile-layout-row kt-row-valign-top\">\n\n<div class=\"wp-block-kadence-column kadence-column1950_b4c2b5-17\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"606\" height=\"766\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/diffusion-cell.jpg\" alt=\"static diffusion measurement\" class=\"wp-image-1923\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/diffusion-cell.jpg 606w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/diffusion-cell-237x300.jpg 237w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/diffusion-cell-500x632.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/diffusion-cell-300x379.jpg 300w\" sizes=\"auto, (max-width: 606px) 100vw, 606px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_6ce61e-1d\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1525\" height=\"1090\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion.jpg\" alt=\"\" class=\"wp-image-1926\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion.jpg 1525w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-300x214.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-1024x732.jpg 1024w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-768x549.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-500x357.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-800x572.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-1280x915.jpg 1280w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/NaCl-ion-diffusion-850x608.jpg 850w\" sizes=\"auto, (max-width: 1525px) 100vw, 1525px\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-column kadence-column1950_80f9bb-c7\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1523\" height=\"1090\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion.jpg\" alt=\"\" class=\"wp-image-1927\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion.jpg 1523w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-300x215.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-1024x733.jpg 1024w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-768x550.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-500x358.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-800x573.jpg 800w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-1280x916.jpg 1280w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/09\/pH-ion-diffusion-850x608.jpg 850w\" sizes=\"auto, (max-width: 1523px) 100vw, 1523px\" \/><\/figure>\n<\/div><\/div>\n\n<\/div><\/div>\n\n\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-1950_d81f5f-d8\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\"\/><\/div><\/div>\n\n\n\n<h2 style=\"text-align: center;\">3D culture need bioreactors<\/h2>\n\n\n\n\n\n<figure class=\"wp-block-kadence-image kb-image1950_c5ae72-8e size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"820\" height=\"317\" src=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section.jpg\" alt=\"GO3D Perfusion Bioreactor Section\" class=\"kb-img wp-image-1931\" srcset=\"https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section.jpg 820w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section-300x116.jpg 300w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section-768x297.jpg 768w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section-500x193.jpg 500w, https:\/\/biomed-center.com\/wp-content\/uploads\/2025\/10\/GO3D-Perfusion-Section-800x309.jpg 800w\" sizes=\"auto, (max-width: 820px) 100vw, 820px\" \/><\/figure>\n<style 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